Locked Nucleic Acid Biomolecular Handles Functionalize Double-Stranded DNA for Single Molecule Manipulation
نویسندگان
چکیده
منابع مشابه
Locked nucleic acid oligomers as handles for single molecule manipulation
Single-molecule manipulation (SMM) techniques use applied force, and measured elastic response, to reveal microscopic physical parameters of individual biomolecules and details of biomolecular interactions. A major hurdle in the application of these techniques is the labeling method needed to immobilize biomolecules on solid supports. A simple, minimally-perturbative labeling strategy would sig...
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Single-molecule imaging of biological macromolecules has dramatically impacted our understanding of many types of biochemical reactions. To facilitate these studies, we have established new strategies for anchoring and organizing DNA molecules on the surfaces of microfluidic sample chambers that are otherwise coated with fluid lipid bilayers. This previous work was reliant upon the use of doubl...
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We have worked out a procedure for covalent binding of DNA to polystyrene microspheres for optical tweezers experiments in aqueous buffer solutions. These new coupling procedures allow preparation of DNA-microspheres in advance and storage of these beads for months. The covalent coupling of the DNA fragments facilitates the investigation of short DNA fragments (<1000 bp) and the rotationally co...
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The polymerization of RecA on individual double-stranded DNA molecules is studied. A linear DNA (lambda DNA, 48.5 Kb), anchored at one end to a cover glass and at the other end to an optically trapped 3-micrometers diameter polystyrene bead, serves as a template. The elongation caused by RecA assembly is measured in the presence of ATP and ATP[gammaS]. By using force extension and hydrodynamic ...
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During the past decade, local force measurement techniques, such as atomic force microscopy and optical tweezers, were used to study the elastic properties and mechanically induced structural transitions of nucleic acids at the single-molecule level. Single-molecule manipulation has also increasingly been used to investigate DNA-dependent enzymatic processes, with implications for unfolding and...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2013
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2012.11.2856